Stem Cell Quality Control Services and Reagents
Cytogenetic and genomic services on stem cells including karyotyping, FISH, CNV, LOH, gDNA-seq, RNA-seq, etc. are available through our partners at Storrs. Other quality control services and reagents are available at the UConn Stem Cell Core for internal users as follows:
- Immunostaining Kit: This is used to detect pluripotency markers including cell surface markers, e.g., SSEA3, SSEA4, TRA1-60, and TRA1-81, and intracellular markers, e.g., NANOG if no exogenous NANOG was introduced to the cells for reprogramming. The Stem Cell Core will provide you with aliquots of the antibodies as well as protocols so you don’t have to purchase each antibody just for the rare use in your lab.
- Pluripotency Marker Primer Kit: This is used to determine expression of endogenous pluripotency marker genes including OCT4, NANOG, SOX2, LIN28, KLF4, and c-MYC. The Stem Cell Core will provide you with aliquots of the primers as well as protocols.
- Pluripotency Assay via Teratoma Formation: To test pluripotency in vivo, cells are injected into immunity-deficient mice. Any tumors that form are analyzed to look for tissues representing all the three germ layers. The Stem Cell Core will perform this service for you.
- Mycoplasma Testing: Performed using the MycoAlert kit from Lonza.
- Stem Cell Culture Reagents:
- Knockout Serum Replacement (KOSR)
- Mouse Embryonic Fibroblasts (MEFs)