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UConn Health University of Connecticut-Wesleyan University Stem Cell Core

Karyotype Analysis of G-banded Metaphase Chromosomes

Karyotype Abnormalities
Karyotype abnormalities may be observed with long-term culture of cell lines, including trisomy 12and trisomy 17.

Karyotype analysis of G-banded metaphase chromosomes will detect both numerical and structural chromosome abnormalities. This is a gross level (5-10 Mb resolution), first pass method for determining the integrity of your cell line.

When?

  • Upon establishment of a new cell line
  • Every 10 passages
  • After experimental manipulation
  • Prior to publication
  • Prior to banking in a cell repository

How?

Cells should be submitted for karyotyping one day before the culture would normally be passaged to provide for a sufficient mitotic index (number of dividing cells). Analysis of human metaphase chromosomes is done using standard protocols for chromosome harvesting and slide preparation. For each cell line submitted, a minimum of 20-cells will be counted and sexed, and five cells completely analyzed. A karyogram will be produced for two cells per clonal line. Additional counts will be performed in cases of suspected mosaicism.

For further information regarding the basics of karyotyping and how it is used to detect chromosome abnormalities, please visit Karyotyping for Chromosomal Abnormalities.